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. 2017 Dec 13;4:197. doi: 10.3389/fvets.2017.00197

Figure 1.

Figure 1

Production of cytokines in S2308 and 2308ΔOmp25-infected HPT-8 cells. HPT-8 cells were infected with S2308 or 2308ΔOmp25 at a 100:1 MOI. Control group was uninfected. At 4, 8, 24, and 48 h post-infection, supernatant samples were collected and TNF-α (A), IL-1 (B), and IL-10 (C) levels were assayed by ELISA. Cytokines production is expressed as the mean cytokine concentration ± SD for each group of cells. Significant differences between the S2308 and 2308ΔOmp25 are indicated by * (P < 0.05). OMP25, outer membrane protein 25; IL-1, interleukin-1; IL-10, interleukin-10; MOI, multiplicity of infection; TNF-α, tumor necrosis factor-α.