Figure 3.

Activation of p38, ERK1/2, and JNK kinases in S2308 and 2308ΔOmp25-infected HPT-8 cells. HPT-8 cells were infected with S2308 or 2308ΔOmp25 at a 100:1 MOI. At 24 h post-infection, supernatant was discarded, and cells were lysed. Then, the activation of p38, ERK1/2, and JNK kinases was detected by Western blotting. Infections with 2308ΔOmp25 induced a markedly weaker stimulation of p38, ERK1/2, and JNK kinases, with S2308 demonstrating a slightly higher capacity of activation than 2308ΔOmp25. OMP25, outer membrane protein 25; JNK, Jun-N-terminal kinase; MOI, multiplicity of infection.