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. 2017 Dec 13;4:197. doi: 10.3389/fvets.2017.00197

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Copyright © 2017 Zhang, Zhang, Li, Liu, Shao, Wu, Wang, Wang, Li, Liu, Chen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production of TNF-α in S2308 and 2308ΔOmp25 vaccinated BALB/c mice. BALB/c mice were inoculated with 1 × 10⁶ CFU of S2308 or 2308ΔOmp25. Control groups received PBS. At 2, 4, 6, 8, and 10 weeks post-immunization, serum samples were collected (n = 5 per time point) and TNF-α levels were assayed by ELISA. TNF-α production is expressed as the mean cytokine concentration ± SD for each group of mice. Significant differences between the S2308 and 2308ΔOmp25 are indicated by * (P < 0.05). OMP25, outer membrane protein 25; TNF-α, tumor necrosis factor-α; PBS, phosphate-buffered saline.