Figure 1.

Caveolin-1 (CAV1) is expressed in dendritic cells (DCs) and upregulated upon maturation. CAV1 expression in DCs was assessed by Western blotting. GAPDH and actin were used as loading controls. CAV1 protein expression was quantified by densitometry analysis and standardized to loading control. Values normalized to untreated controls (NT) are shown. (A) Spleen DCs purified from wild-type mice were treated with LPS (100 ng/ml) for 6 h. (B,C) Bone marrow-derived DCs (BM-DCs) were stimulated with LPS (100 ng/ml) or TNF-α (20 ng/ml) for 0, 1, 3, 6, 12, or 24 h. (D) BM-DCs were treated with LPS (100 ng/ml) alone or in combination with a TNF-α blocking antibody (5 µg/ml) for 24 h. Values are presented as the mean ± SEM. *p < 0.05; n = 3 independent experiments.