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. 2017 Dec 13;8:1794. doi: 10.3389/fimmu.2017.01794

Figure 4.

Figure 4

Caveolin-1 (CAV1) promotes the ability of dendritic cells (DCs) to induce antigen-specific CD8+ T cell responses. (A) Scheme of the experimental procedure. At day 0, 1 × 106 OVA257–265-pulsed wild-type (WT) or CAV1−/− bone marrow-derived DCs (BM-DCs) were transferred to recipient WT mice. After 7 days, blood samples were taken, and CD8+ T cell responses were analyzed. (B) Cells were stimulated with control (trp2180–188) or OVA257–265 peptides for 2 h, and then brefeldin A-containing solution (Golgi plug) was added for another 6 h (8 h total stimulation). Afterward, cells were stained and analyzed by flow cytometry. Representative dot plots of IFN-γ expression on gated CD3++CD8+ T cell population and the percentage of IFN-γ-producing CD8+ T cells are shown. Bars are the mean ± SEM (*p < 0.05, n = 7 mice, from two independent experiments). (C) Freshly isolated cells were stained with H-2 Kb/SIINFEKL (OVA257–265 peptide) dextramer to determine antigen-specific CD8+ T cells. Representative density plots and quantification of frequency of dextramer-positive from totalCD3+CD8+ T cell population are shown. Data are the mean ± SEM (*p < 0.05, n = 5–6 mice from two independent experiments).