Figure 3. TDCIPP-induced dorsalization at 24 hpf is preceded by epiboly arrest at 6 hpf.

(A) Representative images of three epiboly phenotypes (normal, delayed, and arrested) at 6 hpf. Untreated embryos are normally at the germ ring or shield stage by 6 hpf. Epiboly-delayed embryos only progress to the dome and 30% epiboly stage by 6 hpf; epiboly-arrested embryos stall at the high stage by 6 hpf. (B) TDCIPP exposure results in a concentration-dependent increase in epiboly-arrested embryos, whereas DMP-treated embryos show no epiboly defects at 6 hpf. (C) Compared to <10% for normal and delayed embryos, 76% of embryos with epiboly arrest are dorsalized at 24 hpf.