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. 2017 Dec 14;5:e4167. doi: 10.7717/peerj.4167

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©2017 Barwell et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Temporal control of the UAS/GAL4 system by Tet-off GAL80 transgenes. (B) Two different Tet-off GAL80 plasmids were generated with different promoters driving tTA expression: pDJ146 (tubulin 1α) and pDJ147 (actin5C). Block arrows, promoters; Pentagon, endogenous enhancers; Lozenges, P-element inverted repeats; Triangles, insulator sequences; Ovals, proteins; Rectangles, coding sequences or exons; Rounded rectangles, transcription termination sequences; Striped rectangle, pUC8 plasmid sequence.