Table 2. Survey of GAL4 drivers with UAS-lacZ.
Data presented are the average specific activity (ΔmOD562 nm/min/µg protein) plus or minus the standard deviation (n = 5).
| GAL4 driver | −GAL80 | +GAL80 | p-value | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L1 | L3 | EP | LP | L1 | L3 | EP | LP | L1 | L3 | EP | LP | |
| da-GAL4 | 9.5 ± 1.0 | 84.6 ± 18.1 | 79.1 ± 3.7 | 134.3 ± 17.4 | 1.3 ± 0.2 | 23.7 ± 8.1 | 24.7 ± 8.5 | 21.9 ± 7.6 | 6.9E−08 | 2.4E−03 | 1.1E−04 | 4.1E−05 |
| tub-GAL4 | 12.1 ± 3.6 | 136.3 ± 30.4 | 113.5 ± 14.5 | 165.3 ± 15.9 | 1.4 ± 0.3 | 30.1 ± 3.6 | 35.2 ± 15.8 | 26.6 ± 10.7 | 7.7E−05 | 2.7E−05 | 1.9E−05 | 1.1E−07 |
| 24B | 3.1 ± 0.6 | 22.2 ± 2.9 | 29.3 ± 5.6 | 34.1 ± 6.2 | 0.3 ± 0.2 | 13.1 ± 5.3 | 11.8 ± 4.7 | 36.8 ± 5.7 | 2.8E−06 | 5.0E−03 | 3.5E−04 | 2.4E−01 |
| ELAV | 1.1 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 | 1.2 ± 0.3 | 0.3 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.1 | 1.1 ± 0.2 | 4.6E−08 | 2.0E−03 | 1.1E−01 | 2.2E−01 |
| DJ752 | 2.2 ± 0.7 | 15.6 ± 5.7 | 20.8 ± 4.3 | 9.1 ± 1.5 | 0.2 ± 0.1 | 2.4 ± 1.2 | 1.3 ± 0.7 | 1.7 ± 0.3 | 7.3E−05 | 5.2E−04 | 4.0E−06 | 0.0E+00 |
| DJ761 | 2.5 ± 0.6 | 83.8 ± 11.1 | 47.1 ± 8.6 | 33.8 ± 4.1 | 1.4 ± 1.1 | 8.3 ± 2.6 | 2.7 ± 0.5 | 5.4 ± 1.0 | 3.4E−02 | 2.2E−07 | 1.5E−06 | 1.7E−07 |
| DJ755 | 4.3 ± 1.4 | 12.9 ± 3.3 | 16.6 ± 6.1 | 12.0 ± 4.1 | 0.6 ± 0.2 | 2.0 ± 0.8 | 2.3 ± 0.2 | 1.7 ± 0.3 | 2.4E−04 | 4.5E−05 | 1.6E−04 | 8.5E−05 |
| DJ756 | 5.7 ± 1.8 | 18.1 ± 4.8 | 19.1 ± 6.4 | 8.7 ± 1.9 | 0.3 ± 0.1 | 1.9 ± 1.0 | 1.7 ± 1.1 | 2.3 ± 0.9 | 3.2E−04 | 3.8E−05 | 1.7E−04 | 5.9E−05 |
| DJ1007 | 5.6 ± 2.1 | 7.8 ± 4.1 | 5.5 ± 1.3 | 5.5 ± 1.8 | 1.6 ± 0.9 | 0.8 ± 0.4 | 1.6 ± 0.6 | 1.6 ± 0.4 | 2.0E−03 | 2.5E−03 | 1.3E−04 | 9.2E−04 |
| DJ1040 | 5.9 ± 1.1 | 4.8 ± 2.6 | 8.0 ± 2.0 | 5.1 ± 0.8 | 0.8 ± 0.4 | 0.9 ± 0.4 | 1.9 ± 0.2 | 2.6 ± 1.3 | 3.1E−06 | 5.7E−03 | 6.8E−05 | 2.6E−03 |
| D42 | 2.0 ± 0.2 | 11.9 ± 2.8 | 6.4 ± 3.4 | 2.4 ± 0.4 | 0.3 ± 0.1 | 1.1 ± 0.7 | 0.8 ± 0.4 | 0.6 ± 0.1 | 4.7E−08 | 1.6E−05 | 4.1E−03 | 8.6E−06 |
| DJ817 | 3.7 ± 0.8 | 9.2 ± 4.8 | 10.5 ± 1.2 | 4.1 ± 1.8 | 0.2 ± 0.1 | 0.9 ± 0.5 | 0.6 ± 0.5 | 0.7 ± 0.7 | 5.7E−06 | 2.6E−03 | 7.7E−08 | 1.3E−03 |
| DJ634 | 0.3 ± 0.1 | 20.4 ± 4.1 | 23.5 ± 5.5 | 48.3 ± 10.1 | 0.1 ± 0.1 | 2.7 ± 0.7 | 1.1 ± 0.8 | 0.6 ± 0.2 | 2.1E−04 | 5.6E−06 | 8.7E−06 | 2.7E−06 |
| DJ715 | 2.7 ± 0.4 | 23.8 ± 0.9 | 17.7 ± 5.4 | 15.2 ± 0.1 | 1.3 ± 2.1 | 1.9 ± 0.1 | 2.1 ± 0.1 | 0.4 ± 0.1 | 8.7E−02 | 9.7E−06 | 1.8E−04 | 5.0E−05 |
| DJ695 | 0.2 ± 0.1 | 7.1 ± 2.1 | 9.7 ± 5.2 | 2.8 ± 1.1 | 0 ± 0 | 1.1 ± 0.3 | 0.5 ± 0.3 | 0.3 ± 0.1 | 3.0E−05 | 9.1E−05 | 2.3E−03 | 3.9E−04 |
| DJ946 | 3.3 ± 1.0 | 24.8 ± 13.2 | 7.7 ± 2.6 | 7.9 ± 1.4 | 0.1 ± 0.1 | 0.6 ± 0.4 | 0.3 ± 0.1 | 0.2 ± 0.1 | 4.1E−05 | 1.7E−03 | 1.2E−04 | 8.8E−07 |
| DJ785 | 1.2 ± 0.6 | 17.3 ± 2.2 | 17.1 ± 7.1 | 2.6 ± 1.6 | 0.4 ± 0.4 | 2.4 ± 1.7 | 0.4 ± 0.4 | 0.2 ± 0.1 | 1.0E−02 | 9.4E−07 | 4.0E−04 | 4.3E−03 |
| DJ849 | 0.4 ± 0.1 | 19.3 ± 5.2 | 12.1 ± 1.8 | 1.2 ± 0.4 | 0.1 ± 0.1 | 1.5 ± 0.5 | 0.6 ± 0.3 | 0.2 ± 0.1 | 8.7E−06 | 3.2E−05 | 3.2E−07 | 3.0E−04 |
| ddc-GAL4 | 1.2 ± 0.4 | 2.2 ± 1.1 | 5.9 ± 3.3 | 9.2 ± 4.4 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 | 2.7 ± 1.6 | 1.6E−04 | 1.4E−03 | 3.1E−03 | 7.3E−03 |
| DJ1027 | 2.9 ± 2.1 | 8.6 ± 8.0 | 8.6 ± 5 | 5.1 ± 4.8 | 0.7 ± 0.3 | 0.3 ± 0.4 | 2.3 ± 0.4 | 1.6 ± 1.5 | 6.7E−02 | 2.5E−02 | 1.1E−02 | 8.3E−02 |
| DJ628 | 0.9 ± 0.6 | 27.7 ± 6.5 | 11.9 ± 5.9 | 36.5 ± 22.1 | 0 ± 0 | 5.2 ± 1.5 | 3.4 ± 2.1 | 2.6 ± 1.5 | 3.9E−03 | 3.4E−05 | 8.2E−03 | 4.4E−03 |
| DJ646 | 0.4 ± 0.1 | 12.4 ± 3.2 | 7.9 ± 3.3 | 0.7 ± 0.3 | 0.1 ± 0.1 | 0.5 ± 0.3 | 0.2 ± 0.1 | 0.1 ± 0.1 | 1.4E−04 | 1.8E−05 | 3.7E−04 | 1.4E−03 |
| GAL4.109 | 0.5 ± 0.1 | 12.5 ± 2.2 | 8.3 ± 2.6 | 11.9 ± 2.1 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.5 ± 0.2 | 0.8 ± 0.2 | 2.2E−06 | 7.3E−07 | 9.0E−05 | 1.0E−06 |
| Nrv2-GAL4 | 0.3 ± 0.1 | 1.0 ± 0.2 | 1.5 ± 0.3 | 6.0 ± 1.3 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 1.6 ± 0.7 | 2.3E−03 | 4.7E−05 | 5.9E−06 | 9.0E−05 |
| DJ710 | 0.5 ± 0.1 | 11.2 ± 5.4 | 6.8 ± 5.8 | 0.5 ± 4.7 | 0.4 ± 0.2 | 0.1 ± 0.6 | 0.1 ± 1.0 | 0.1 ± 0.1 | 1.0E−01 | 2.1E−09 | 1.2E−02 | 1.2E−05 |
| cha-GAL4 | 0.5 ± 0.1 | 0.7 ± 0.2 | 0.3 ± 0.1 | 1.4 ± 0.4 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.8 ± 0.2 | 1.1E−03 | 4.9E−05 | 1.8E−02 | 7.7E−03 |
Notes.
- −GAL80
- positive control genotypes (driver + UAS-lacZ); +GAL80, experimental genotypes (driver + UAS-grim + 3.3,DJ1077)
- L1
- first instar larvae
- L3
- third instar larvae
- EP
- early pupae
- LP
- late pupae
p-values represent comparisons between animals with or without GAL80, significant values (p < 0.05) are highlighted in grey.