REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Polyclonal Rabbit anti-mouseIRE1α | Bertolotti et al., 2000 | NY200 |
Polyclonal Chicken anti-hamsterBiP | Avezov et al., 2013 | Anti-BiP |
FLAG-M2 | Sigma | Cat no: F1804 RRID:AB_262044 |
Polyclonal Rabbit anti-GST | Ron and Habener, 1992 | Anti-CHOP |
Polyclonal Rabbit anti-mousePERK-P | Bertolotti et al., 2000 | NY97 |
Polyclonal Rabbit anti-mousePERK | Bertolotti et al., 2000 | NY201 |
Bacterial and Virus Strains | ||
BL21 C3013 E. coli | NEB | Cat no: C3013I |
Origami B(DE3) E. coli | Novagen/MERCK | Cat no: 70837 |
Chemicals, Peptides, and Recombinant Proteins | ||
Thapsigargin | Calbiochem | Cat no: 586005 |
Tunicamycin | Melford | Cat no: T2250 |
2-Deoxyglucose | Sigma | Cat no: D6134 |
4μ8c | Tocris Bioscience | Cat no: 4479 |
Hexokinase | Sigma | Cat no: H4502 |
Phos-tag acrylamide | Distributed by Wako Pure Chemical Industries Synthesized by NARD institute |
NARD: AAL-107 Wako cat: 300-93523 |
Digitonin | Calbiochem | Cat no: 300410 |
Biotin-maleimide | Sigma | Cat no: B1267 |
Oregon Green-iodoacetic acid | ThermoFisher | Cat no: O6010 |
TAMRA-maleimide | Sigma | Cat no: 94506 |
Biotin-NHS ester | Sigma | Cat no: H1759 |
Phosphocreatine | Sigma | Cat no: 10621714001 |
Biotin | Thermo Scientific | Cat no: B1595 |
Creatine kinase | Sigma | Cat no: C3755 |
Experimental Models: Cell Lines | ||
Clone S21 a derivative of RRID: CVCL_0214 | Sekine et al., 2016 | CHO S21 |
Oligonucleotides | ||
See Table S2 for Oligonucleotides | N/A | N/A |
Recombinant DNA | ||
See Table S1 for Recombinant DNA | N/A | N/A |
Software and Algorithms | ||
FlowJo,LLC, | RRID: SCR_008520 | |
GraphPad-Prism V7 | RRID: SCR_002798 | |
ImageJ | RRID: SCR_003070 | |
Other | ||
Octet RED96 | Pall ForteBio | |
1200 Infinity (HPLC) | Agilent technologies | |
Aktapurifier (FPLC) | GE healthcare | |
CLARIOstar platereader | BMG Labtech |