Table 2.
Sequencing depth and coverage in three samples with discrepant results in human leukocyte antigen (HLA) interpretation by the Omixon Explore software.
| Exons covered | Depth range | Exon 1 | Exon 2 | Exon 3 | Exon 4 | Exon 5 | Exon 6 | Exon 7 | Omixon Target | Omixon Explore | Reference method | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| All samples HLA-DQB1 | 5 | 3–279 | 37–305 | 3–105 | 51–446 | 40–307 | 155–413 | na | na | |||
| FRC13 | ||||||||||||
| HLA-DQB1 allele 1 | 4 | Average 134 | 167 | 89 | 148 | 178 | – | na | na | 02:01 | 02:01 | 02:01 |
| HLA-DQB1 allele 2 | 4 | Average 138 | 167 | 89 | 148 | 178 | – | na | na | 02:06 | 02:02 | 02:02 |
| FRC36 | ||||||||||||
| HLA-DQB1 allele 1 | 4 | Average 173 | 189 | 77 | 245 | 205 | – | na | na | 03:01 | 03:01 | 03:01 |
| HLA-DQB1 allele 2 | 2 | Average 118 | – | 77 | 158 | – | – | na | na | 03:22 | 03:22 | 03:01 |
| All samples HLA-A | 7 | 16–322 | 4–206 | 5–239 | 4–305 | 4–463 | 12–426 | 1–389 | 1–366 | |||
| FRC37 | ||||||||||||
| HLA-A allele 1 | 6 | Average 322 | 206 | 239 | 305 | 463 | 426 | – | 1 | 02:01 | 02:01 | 02:01 |
| HLA-A allele 2 | 6 | Average 322 | 206 | 239 | 305 | 463 | 426 | – | 1 | 02:197 | 02:01 | 02:01 |
Samples FRC13 and FRC36 had an HLA-DQB1 discrepancy and sample FRC37 had an HLA-A discrepancy.