FIGURE 2.

Requirement of the adenine species at the transcription initiation base for positive stringent transcription control of P_kinB. The P_kinB regions of nt –75/+10 and –55/+10 were fused with lacZ to yield strains FU1191 P_kinB (–75/+10) and FU1115 P_kinB (–55/+10). The adenine at nt +1 was replaced with a guanine to yield strains FU1193 P_kinB (–75/+10 A+1G) and FU1116 P_kinB (–55/+10 A+1G). The synthesis of β-Gal encoded by lacZ in strains FU1191 and FU1115, and strains FU1193 and FU1116 was monitored during sporulation in a nutrient sporulation medium, NSMP (A,B), and after addition of decoyinine to the culture in minimal medium, S6 (C,D). Circles and squares indicate the P_kinB with adenine and guanine at nt +1, respectively. β-Gal synthesis during sporulation in NSMP was indicated by closed symbols. In the case of S6 medium, closed and open symbols indicate with and without addition of decoyinine, respectively. Large and small symbols denote β-Gal activity and OD₆₀₀, respectively. In all Figures of β-Gal monitoring, the standard deviations of the average β-Gal activity values from the multiple replicates are indicated by error bars (one experiment gives two activity values at an indicated time); tiny error bars are invisible due to their overlap with the symbols. In the case of β-Gal monitoring shown in (A,B), the experiments were performed with triple replicates.