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. 2017 Dec 13;8:2502. doi: 10.3389/fmicb.2017.02502

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Copyright © 2017 Fujita, Ogura, Nii and Hirooka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Truncation and deletion analyses of the P_kinB region to identify a repressor-binding site. (A) Truncation analysis. β-Gal synthesis in strains FU1115 P_kinB (–55/+10) (red circles), FU1190 P_kinB (–65/+10) (brown circles), FU 1191 P_kinB (–75/+10) (squares), FU1192 P_kinB (–85/+10) (triangles), and FU1182 P_kinB (–95/+10) (diamonds) was monitored during sporulation in NSMP medium and after addition of decoyinine to S6 medium. (B) Inner deletion analysis. β-Gal synthesis in strains FU1115 P_kinB (–55/+10) (circles), FU 1191 P_kinB (–75/+10) (squares), FU1195 P_kinB (–75/+10 Δ5)(triangles), and FU1196 P_kinB (–75/+10 Δ10) (diamonds) was monitored during sporulation in NSMP medium and after addition of decoyinine to S6 medium.