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Electrophoretic mobility shift assay analysis of SinR binding to the mutant PkinB probes. The wild-type probes [PkinB (–124/–38) ( = Rm0) and PkinB (–39/+104) ( = Fm0)], as well as the mutant ones carrying the respective three-base substitutions in the SinR-1 site (Rm1, Rm2, Rm3, Rm4, Rm5, and Rm6) and in the SinR-2 site (Fm1, Fm2, Fm3, Fm4, Fm5, and Fm6) were prepared using the wild-type and mutant primer pairs and a DNA template (Supplementary Tables S1, S2-2).
