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Competition binding curves at the A1AR, A3AR, and β2AR for three hits identified from the LOPAC library. CHO cell lines stably expressing A1AR (A), A3AR (B), or β2AR (C) were incubated with 25 nM CA200645 (A3AR and A1AR) or 10 nM BODIPY-TMR-CGP (β2AR) in the absence or in the presence of increasing concentrations of the indicated compounds. Values are mean ± SEM from three independent experiments performed in triplicate.
