Figure 1.
Myc and YAP coregulate cell cycle entry. Serum-starved subconfluent (sparse) (A) or highly confluent (confluent) 3T9MycER;YAP (B–H) cells were treated with OHT to activate MycER and doxycycline (dox) to trigger the expression of YAPS127A. (A) Cell cycle entry was measured by immunofluorescence analysis of EdU incorporation. DAPI was used to color nuclei. (B) Ranked heat map based on the log2 fold change of the differentially expressed genes (DEGs) identified by RNA sequencing (RNA-seq). (C) Box plot of the mRNA expression level of the Myc-dependent serum response (MDSR) genes (D) Gene ontology map based on the DEGs determined upon both MycER activation and YAP induction. (E) Cumulative bar graph of Myc, YAP, and TEAD ChIP-seq (chromatin immunoprecipitation [ChIP] combined with high-throughput sequencing) peaks, color-coded based on their overlap. (Y) YAP; (M) Myc; (T) TEAD. (F,G) Box plot of the enrichment of Myc (F) and YAP (G) ChIP-seq peaks divided into subsets as in E. (H) Expression levels of up-regulated genes cobound at their promoters by YAP, Myc, and TEAD (YMT peaks).