Figure 3.

Cooperative binding and transcriptional activation by Myc, TEAD, and YAP. Genome-wide analyses of livers from R26-rtTA mice either wild type (wt), transgenics for Myc (tet-Myc) or YAP (tet-YAP), or double transgenics (tet-Myc/YAP). Short-term induction was achieved by feeding mice with doxycycline-containing food for 48 h. (A) Liver sections stained with an anti-Ki67 antibody. (B) Hierarchical clustering of DEGs. (C) Box plot showing a representative cluster of YAP/Myc DEGs. (D) Venn analysis of Myc, YAP, and TEAD ChIP-seq peaks. The number of peaks determined for each TF is reported in brackets; the arrows point to the number of overlapping peaks. (E) Ranked heat maps of the ChIP-seq enrichment of the indicated TFs. (Top panel) YAP peaks detected only in tet-YAP livers. (Bottom panel) Promoters bound by YAP in tet-Myc/YAP livers. (F,G) H3K27ac (F) and H3K4me3 (G) levels at promoters of DEG-up genes cobound by Myc and YAP.