Figure 5.

Targeting AdipoR1 signaling in SET1B-expressing triple-negative breast cancer. (A) MDA-MB-231, MDA-MB-468, and LM2 cells were seeded in six-well plates at a concentration of 200 cells per well. The cells were treated with either DMSO or 10 µM AdipoRON for 3 wk, and the colonies were stained with crystal violet. (B) Quantification of crystal violet staining was determined as described in the Materials and Methods. n = 4. (**) P < 0.01, Student's t-test was used for statistical analysis. Error bars represent SD. (C) Flow chart of the animal experiments in D–F. (D) LM2 cells (4 × 10⁶ cells) were inoculated into the fat pads of nude mice. Twelve days after injection, when the tumors reached 100 mm³, mice were divided randomly into two groups. They were treated 10 times with either 50 mg/kg PBS or 50 mg/kg AdipoRon, and the tumor size (D,E) and animal survival (F) were monitored. Tumor growth was measured 2 wk after inoculation. n = 7. (*) P < 0.05, Student's t-test was used for statistical analysis. Log-rank test was used to determine differences between the survival of each group. (G) LM2 cells (4 × 10⁶ cells) were inoculated into the fat pads of nude mice. Twelve days after injection, when the tumors reached 100 mm³, mice were divided randomly into three groups. They were treated 10 times with either 50 mg/kg PBS or 100 mg/kg AdipoRon, and the tumor size was monitored. (H) Tumor growth was measured 2 wk after inoculation. n = 8. (**) P < 0.01; (*) P < 0.05, Student's t-test was used for statistical analysis.