Figure 5.

Biological activities of germicidin A, chalcone, and albaflavenone-hexane extract. Bioassays (A–C) were performed on the six-sector cultivation titration plates in two sets with negative control triplets (see Methods for more details), representative pictures are shown. (A) (Neg.) negative control culture on ONA medium without germicidin A. (Grad.) culture on ONA medium with a linear concentration gradient of germicidin A (0–8 μg mL⁻¹ respectively to the yellow wedge). (B) (Neg.) negative control culture on ONA medium with a DMSO gradient without chalcone. (Grad.) culture on ONA medium with a linear concentration gradient of chalcone (0–8 μg mL⁻¹ respectively to the yellow wedge). (C) (Neg.) negative control culture on ONA medium with a pure hexane gradient. (Grad.) culture on ONA medium with a linear concentration gradient of the albaflavenone-hexane extract respective to the yellow wedge. (D) A dehydrogenase activity test. Spores germinated in the presence of the albaflavenone-hexane extract (red) or in the presence of pure hexane (black). Metabolic activity was measured at denoted time points by optical density at 484 nm.