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. 2017 Dec 13;8:1766. doi: 10.3389/fimmu.2017.01766

Figure 2.

Figure 2

Inhibition of cigarette smoke extract (CSE)-induced CXCL16 expression in arterial endothelial cells. (A) CXCL16 expression was determined by flow cytometry in human umbilical arterial endothelial cells preincubated or not with apocynin (30 µM) or allopurinol (100 µM) for 1 h and then stimulated with 1% CSE for 24 h (n = 7 independent experiments). Results are expressed as mean of fluorescence intensity (MFI). Values are expressed as the mean ± SEM. *P < 0.05 relative to values in the medium group; ++P < 0.01 relative to 1% CSE group. Endothelial cells were transfected with (B) Nox2, (C) Nox4, or (D) Nox5 siRNA or control siRNA. At 48 h post-transfection, cells were stimulated with 1% CSE for 24 h. CXCL16 expression was determined by flow cytometry. Results are expressed as MFI (n = 5–11 independent experiments). Values are expressed as mean ± SEM. *P < 0.05 or ** P < 0.01 relative to values in the medium group; ++P < 0.01 relative to 1% CSE group in control siRNA transfected cells.