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. 2017 Dec 13;8:1766. doi: 10.3389/fimmu.2017.01766

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Copyright © 2017 Marques, Collado, Escudero, Rius, González, Servera, Piqueras and Sanz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Percentage of CXCR6-expressing leukocytes in different circulating subpopulations from active and not active smoking chronic obstructive pulmonary disease (COPD) patients and aged-matched controls by flow cytometry. Heparinized whole blood was co-stained with specific markers for (A,B) neutrophils, (C,D) monocytes, and (E,F) lymphocytes and CXCR6. Blood samples were incubated or not with EDTA. Results are expressed as percentage of positive cells (n = 17 aged-matched controls, n = 17 active smokers COPD patients, n = 16 ex-smokers COPD patients). Values are expressed as mean ± SEM. *P < 0.05 or **P < 0.01 relative to values in the control group.