Table 2.
Conserved nucleotide signature elements of Cyanobacterial tRNAs.
| tRNA Isotypes | 5′ Acceptor Arm (1–7 nt) | D-arm (10–13 nt) | D-loop | AC- arm (27–31 nt) | Anti-codon loop (32–38 nt) | Variable loop | Ψ-arm (49–53 nt) | Ψ-loop (54–60 nt) |
|---|---|---|---|---|---|---|---|---|
| Gly | G1-C2-G3 | ***** | A1-x2-G4-G5 | ***** | U2-x-C4-C5-A6 | ***** | G5 | U1-U2-C3-x-A5 |
| Ala | G1-G2-G3 | G1-C2-U3-C4 | A1-x2-U4-G5-G6-x-A8 | ***** | U2-x-G4-C5-A6 | G5 | G5 | U1-U2-C3-x-A5 |
| Pro | C1-x-G3-G4-x2-G7 | C4 | A1-G2-x6-A9 | ***** | U1-U2-x-G4-G5-G6 | G3-x-C5 | G1-x2-G4-G5 | U1-U2-C3-x-A5-A6-U7 |
| Val | G1-G2-x-C4 | C2-x-C4 | A1-G2-x3-G6-x-U8-A9 | ***** | U1-U2-x-A4-C5-A6 | G3-U4-C5 | G4-G5 | U1-U2-C3-x-A5 |
| Leu | ***** | ***** | A1-A2-x2-G5-G6-x-A8 | ***** | U2-x-A4 | ***** | G5 | U1-U2-C3-x-A5-x-U7 |
| Ile | G1-G2-G3-C4 | G1-C2-U3-C4 | A1-x4-G6-x2-A9 | ***** | C1-U2-x-A4-U5-A6-A7 | G3-U4 | G4-G5 | U1-U2-C3-x-A5-x-U7 |
| Met | ***** | G2 | G6 | ***** | C1-U2-C3-A4-U5-A6-A7 | ***** | G5 | U1-U2-C3-x-A5-x-U7 |
| Phe | C1/G1-C2-C3-x2-G6 | G1-C2-U3-C4 | A1-G2-U3-U4-G5-G6-U7 | G4 | U2-G3-A4-A5-x-A7 | G3-U4-C5 | G5 | U1-U2-C3-x-A5 |
| Tyr | G1-G2-G3-U4-C5 | G1-x-C3-C4 | A1-G2-U3-G4-G5-U6-U7-A8 | ***** | U2-G3-U4-A5 | ****** | G4-G5 | U1-U2-C3-x-A5-x-U7 |
| Trp | G1 | G1-U2 | A1-x3-G5 | G4-U5 | C1-U2-C3-C4-A5-A6-A7 | ****** | G5 | U1-U2-C3-x-A5-x-U7 |
| Ser | G1-G2-A3 | G1-C2 | A2-x2- G5- G6 | ***** | U2-x3-A5-A6 | ****** | A4/G4-G5 | U1-U2-C3-x-A5-x-U7 |
| Thr | G1-C2 | G1-C2-x-C4 | U2-x-G4-U5-A6-A7 | ***** | U2-x-G4-U5-A6-A7 | G3 | G5 | U1-U2-C3-x-A5 |
| Cys | G1 | G1-C2-C3 | A1-A2-G3-x-G5- G6-U7 | ***** | C1-U2-G3-C4-A5-A6-A7 | C5 | G5 | U1-U2-C3-x-A5-x-U7 |
| Asn | U1-C2-C3-x-C5 | G1-C2-U3 | A1-x1−2-G3-G4 | ***** | C1-U2-G3-U4-U5-A6-A7 | G3-U4 | G5 | U1-U2-C3-x-A5-x-U7 |
| Gln | U1-G2-x3−4-G6 | **** | A1/U1-A2-x2-G4 | ***** | U1-U2-U3-U4-G5 | ***** | A3-G4-G5 | U1-U2-C3-x-A5 |
| Lys | G1-G2-G3 | C2 | A1-x2- G4-G5 | ***** | C1-U2-U3-U4-U5-A6-A7 | ***** | G4-G5 | U1-U2-C3-x-A5-x-U7 |
| His | G1-C2/U2-G3-x3-G7 | G1-C2-C3 | A1-A2-G3-x- G5- G6-U7 | G4-G5 | U1-U2-G3-U4-G5 | ***** | G1-x-G3-G4-G5 | U1-U2-C3-x-A5-x-U7 |
| Arg | G1-G2 | G1 | A1-x2−3-G4-x3-A8 | **** | U1/ C1-U2-x-C4 | ***** | G4-G5 | U1-U2-C3-x-A5-x-U7 |
| Asp | G1-G2-G3 | G1-x-U3-C4 | A1-x2−3-G4- G5-U6-x-A8 | **** | C1-U2-G3-U4-C5-A6 | A1-x-G3-U4 | G1-x-G3-x-G5 | U1-U2-C3-x-A5-x-C7 |
| Glu | G1 | **** | G5 | **** | U2-U3-U4-C5 | **** | G3-x-G5 | U1-U2-C3-x-A5-x-U7 |
The asterisk (*) mark represent the absence of conserved nucleotide consensus sequence in respective region of the tRNA.