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. 2018 Jan;24(1):6–11. doi: 10.1261/rna.063891.117

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© 2018 Naruse et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — ATPase activities of Hsp90β and Hsc70 are necessary for RISC assembly initiated with small RNA duplex but not with single-stranded RNA. (A) Coomassie staining of recombinant ATPase mutants of Hsp90β and Hsc70. (B–D) Target RNA cleavage assay by reconstituted human Ago2-RISC using ATPase mutants of Hsp90β and Hsc70 with let-7 siRNA duplex (B), 9GU wobble duplex (C), and single-stranded RNA (D). (E) Quantification of target cleavage in B–D. The mean ± SD from three independent experiments is shown.