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. 2018 Jan;24(1):6–11. doi: 10.1261/rna.063891.117

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© 2018 Naruse et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Dicer/TRBP is dispensable for RISC assembly in the reconstituted system. (A) Coomassie staining of recombinant Dicer and TRBP proteins. (B) Dicing assay by recombinant Dicer/TRBP. (C) Target RNA cleavage assay by human Ago2-RISC programmed in the presence or absence of Dicer/TRBP. Ago2 was immunopurified from Dicer1^−/− MEF cells. Supplementation of Dicer/TRBP did not enhance RISC assembly in the reconstituted system. (D) Quantification of target cleavage in C. The mean ± SD from three independent experiments is shown.