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. 2018 Jan;24(1):77–89. doi: 10.1261/rna.062927.117

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© 2018 Vincent et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

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FIGURE 6. — Some novel SPAF subcomplexes persist in the absence of RNA. (A) Benzonase treatment of yeast extract confirms degradation of RNA. Total RNA was isolated from YPH499, treated with or without Benzonase, and run on a 1% (w/v) TAE gel. (−B) No Benzonase; (+B) Benzonase treated. The mature rRNAs, 18S and 25S, are indicated in the untreated lane. (B) Some SSU processome subcomplexes do not change sedimentation profile after treatment with Benzonase. Glycerol-gradient analysis of the tagged proteins as in Figure 5. (−) No Benzonase; (+) Benzonase treated. (C) A Nop9-containing subcomplex is released from the SSU processome after treatment with Benzonase. Glycerol-gradient analysis of tagged Nop9 as in Figure 5. (−) No Benzonase; (+) Benzonase treated.