Figure 3.

Comparison of centrosome MTOCs with EGFP-F3-AKAP350A-induced MTNCs. A, HeLa cells transfected with EGFP-F3-AKAP350A were treated with 33 μm nocodazole for 2 h at 37 °C to depolymerize microtubules, washed with DMEM, and allowed to recover for 10 or 20 min to initiate microtubule recovery, fixed in methanol, and stained for α-tubulin. Bar, 10 μm. B, formation of microtubules asters was quantified using ImageJ particle analysis. After 10 min of nocodazole recovery, the average size of asters originating from centrosome MTOCs sizes was 2.00 ± 0.90 μm² (n = 54) versus 1.43 ± 0.85 μm² for induced MTNCs (n = 177) (*, p = 0.0001); after 20 min of nocodazole recovery, the average centrosome MTOC aster size was 5.08 ± 3.9 μm² (n = 71) versus 3.36 ± 2.43 μm² for induced MTNCs (n = 79) (**, p = 0.0019). (Data are presented as average ± S.D.).