Figure 5.

The increased NKG2DL expression by fibroblasts after viral infection does not depend on interferon α/βs. A, fibroblasts were cultured with vehicle, IFNα (1000 units/ml), or IFNβ (1000 units/ml) overnight and then qRT-PCR was used to assay MICA, ULBP2, and IFI16 expression (3 experiments). B, fibroblasts were either left uninfected or infected with the VVΔE3L virus in the presence or absence of 5 μg/ml of the MMHAR2 antibody that blocks the interaction of α/β interferons with their receptor. qRT-PCR was used to assay MICA, ULBP2, and IFI16 expression (6 experiments). ***, p < 0.001.