Figure 7.

Phosphorylation of lipin 1 (3PBD) at Ser¹⁰⁶. HeLa cells infected with adenovirus expressing either FLAG-lipin 1 or FLAG-lipin 1 (3PBD) were treated with vehicle or Torin 1, harvested, and incubated with anti-FLAG beads. The enzymes were displaced, separated on SDS-polyacrylamide gel, and probed for phospho-Ser¹⁰⁶ or total lipin protein. A, image of phosphorylated Ser¹⁰⁶ and total enzyme. B, quantitation of three independent experiments. C (left), Coomassie image of purified phosphorylated lipin 1 and lipin 1 (3PBD); right, Coomassie image of purified phosphorylated lipin 3 and lipin 3 (1PBD). Shown is a scatter plot with mean of the quantitation of three independent experiments. One-way ANOVA was used to analyze statistical significance followed by Sidak post hoc analysis. *, p < 0.05. Error bars, S.E.