Figure 5.

ΔCTL becomes more bundled with time. Electron micrographs of polymers formed by FtsZ or ΔCTL spotted on grids at the indicated amount of time after addition of nucleotide and stained with uranyl formate. A, 4 μm FtsZ with 2 mm GTP, 50 mm KCl, and 10 mm MgCl₂. B, 4 μm ΔCTL with 2 mm GTP, 50 mm KCl, and 10 mm MgCl₂. C, 4 μm FtsZ with 0.2 mm GMPCPP, 50 mm KCl, and 10 mm MgCl₂. D, 4 μm ΔCTL with 0.2 mm GMPCPP, 50 mm KCl, and 10 mm MgCl₂. Scale bars, 100 nm. *, single protofilaments; arrow, two- or three-filament bundles; #, multifilament bundles.