Figure 1.

1,25D treatment increases mRNA levels of CD274 in epithelial cells and of CD274 and CD273 in myeloid cells. A–C, analysis by RT-qPCR of the regulation by 100 nm 1,25D of CD274 and CD273 gene expression in SCC25 and SCC4 cells (epithelial) and THP-1 cells (myeloid) (A), primary human keratinocytes (B), and primary human macrophages (C). -Fold change and p values are relative to control sample (C or 0 h) and are calculated separately for each gene (CD274 and CD273). C, vehicle (ethanol); D, 100 nm 1,25D (n = 3); ns > 0.05 ≥ * ≥ 0.01 ≥ ** ≥ 0.001 ≥ ***. Error bars, S.D.