Figure 3.

Interaction of different ERS with Erd1 and Erd2. A–D, under conditions with or without overexpressed Erd1 or Erd2, the SD efficiencies of the Aga2–GFP–FLAG (A) or Aga2–GFP–FLAG–ERS cassettes (B–D) (ERS: HDEL, FEHDEL, or WEHDEL) were recorded at 24 h after induction at 30 °C. E, the mRNA levels of ERD1 and ERD2 in the cells bearing Aga2–GFP–FLAG or Aga2–GFP–FLAG–WEHDEL cassettes were quantitated by RT-PCR after induction for 6 h at 30 °C. The cells were surface-labeled with iFluor 647–conjugated anti-FLAG antibodies for SD efficiency quantitation. The data are presented as mean ± S.E. (n = 3 independent experiments). p ≤ 0.05 (Student's t test).