Table 1.
Proteins containing carboxyl-terminal sequence of DEL in S. cerevisiae
The sequences of 15 proteins from S. cerevisiae were extracted from the NCBI database. The italics and underlined letters represent the common sequence of HDEL motif ERS. Information regarding functional properties and localization was adapted from http://www.yeastgenome.org/. (Please note that the JBC is not responsible for the long-term archiving and maintenance of this site or any other third party hosted site.)
| C-terminal sequences | Genes | Function | Location | Strength of ERSa |
|---|---|---|---|---|
| NFRDEL | SIL1 (Ref. 19) | Nucleotide exchange factor for the ER luminal chaperone Kar2p | ER | Medium |
| DGEDEL | GPI17 (Ref. 21) | Subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins | ER membrane | Weak |
| PYLDEL | QCR2 (Ref. 32) | Subunit 2 of ubiquinol cytochrome-c reductase | Mitochondrion inner membrane | Weak |
| MLKDEL | SCJ1 (Ref. 20) | A homolog of bacterial chaperone DnaJ, cooperates with Kar2p to mediate maturation of proteins | ER lumen | Medium |
| AIHDEL | PDI1 (Ref. 33) | Protein disulfide isomerase, essential for disulfide bond formation in secretory and cell-surface proteins | ER lumen | Strong |
| GLHDEL | SED4 (Ref. 34) | Integral ER membrane protein that stimulates Sar1p GTPase activity | ER membrane; Golgi apparatus membrane | Medium |
| AAHDEL | CPR5 (Ref. 35) | Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues | ER lumen | Weak |
| TVHDEL | EUG1 (Ref. 36) | Protein disulfide isomerase of the ER lumen | ER lumen | Medium |
| VSHDEL | SEC20 (Ref. 37) | Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to ER | ER | Medium |
| ILHDEL | LHS1 (Ref. 38) | Molecular chaperone of the ER lumen; involved in polypeptide translocation and folding | ER lumen | Strong |
| SSHDEL | MPD2 (Ref. 39) | Member of the Pdi family; exhibits chaperone activity | ER | Weak |
| PLHDEL | KRE5 (Ref. 40) | Protein required for β-1,6-glucan biosynthesis | ER lumen | Strong |
| NKHDEL | MPD1 (Ref. 41) | Member of the Pdi family; interacts with and inhibits the chaperone activity of Cne1p | ER lumen | Weak |
| IEHDEL | YOS9 (Ref. 42) | ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins in ERAD | ER membrane | Strong |
| FEHDEL | KAR2 (Ref. 43) | ATPase involved in protein import into the ER; acts as a chaperone to mediate ER protein folding and may help export soluble proteins | ER lumen | Strongb |
a The strength of the ERS was evaluated in our research.
b The high level of ER retention strength in FEHDEL was identified in both our and others' research (14).