Figure 5.
Effect of αL-Phe-153 mutations on αLβ2-mediated cell adhesion and spreading. A, HEK293FT cells transfected with the indicated integrin αLβ2 constructs were seeded onto the plates coated with human ICAM-1-Fc (5 μg/ml coating concentration) at 37 °C for 1 h. The cells were fixed and immunostained with anti-αL mAb TS2/4 shown in green. The nuclei were stained with DAPI. Scale bar, 200 μm. B, flow cytometry plots showing integrin αLβ2 expression reported by mAb TS2/4 in the corresponding HEK293FT transfectants used in A. One representative experiment of more than three repeats is shown.