Figure 6.

NP12 mediates up-regulation of Nanog in infarcted myocardium. a, representative immunofluorescence analysis of β-catenin (green) and CD31 (red) double-labeling in the infarcted myocardium at days (d) 7 and 14, receiving either PBS or NP12 versus sham. b, representative confocal images of double-staining with Nanog (green) with CD31 (for optimal resolution, the red color is converted into white) in the infarcted myocardium at days 7 and 14, receiving either PBS or NP12 versus sham heart. Panels to the right of b show magnified images with yellow arrowheads indicating co-localization of nuclear Nanog with CD31⁺ vascular structures. c–f, mRNAs prepared from CD31⁺ cardiac ECs obtained from sham-, PBS-, and NP12-receiving mice were analyzed by qRT-PCR for expression of Nanog, cyclin-D1, Ki67, and Vegfr2 transcripts using 18S rRNA as internal control. Data show a significant increase in expression of target genes compared with the indicated groups; thereafter, data were subjected to ANOVA followed by Sidak's test. We used n = 3 mice each group for qRT-PCR analysis, and n = 6 mice each group for immunostaining. Error bars represent ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus indicated groups. ns, not significant.