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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Mol Neurobiol. 2017 Jun 17;55(5):4320–4332. doi: 10.1007/s12035-017-0649-5

Fig. 6.

Fig. 6

Live labeling with sema4D antibody shows change of outer retina sema4D in WT and RCS retina comparing after light onset. a Live sema4D antibody and secondary antibody labeling (shown in red) of whole mount WT retina 1 hour before and after light onset as indicated. b Quantification of signal intensity of images as in a. c Labeling of RCS rat retina as in a. d Quantification of signal intensity of images as in c. Images show representative maximal projections of x-y confocal image stacks obtained in three independent experiments. Scale bars: 10 μm. Bars show mean ± SD of three independent experiments with duplicate samples each. Asterisks indicate significant difference of sema4D levels before and after light onset, p < 0.05 by Student’s t-test.