Fig. 8.

PlxnB1 KO RPE harbors excess POS phagosomes. a Images show comparable retinal histology in PlxnB1 knockout and WT eyes with all retinal layers present: Ganglion cell layer (GCL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); photoreceptor outer segments (POS); RPE. Scale bar: 30 μm. b Representative immunoblot probed for retinal and RPE markers as indicated and discussed in the text. Samples were dissected mouse eyes harvested 1 hour or 8 hours after light onset as indicated. Porin served as loading control. c Representative maximal projections of x-y confocal image stacks of retinal tissue sections labeled with opsin antibody (shown in green, nuclei counterstain shown in blue) obtained from mice sacrificed 1 hour or 8 hours after light onset as indicated. Images were intentionally overexposed to detect POS phagosomes in the RPE (arrow heads) in addition to intact outer segments. Scale bar: 10 μm. d Bars show number of phagosomes per 100 μm RPE in WT (gray bars) and PlxnB1 KO (black bars) mouse eyes harvested 1 hour or 8 hours after light onset. Bars show number of phagosomes counted in 100 μm in each condition, mean ± SD, n = 5 mice per strain. Asterisks indicate significant difference between WT and KO, p < 0.05 by ANOVA.