Figure 2.

Activity of ssRNA on TLR8 stimulation increases with the number of UpG motifs present. The TLR8 activity of G5 was normalized to 1, while the TLR8 activity without RNA (N. R.) has been set as 0. White: G on 3′; Black: G on 5′. All oligos were tested at 5μM on HEK293-TLR8 cells, transfected with DOTAP