FIG. 4.

Complement depletion with CVF results in increased production of chemoattractive chemokines in tumor microenvironment. qRT-PCR performed on tumor tissues of mice treated with PBS or CVF showed significantly higher expression [fold increase over HPRT (hypoxanthine guanine phosphoribosyl transferase) (murine housekeeping gene)] of chemokines CCL5 (0.03-fold vs. 4.3-fold; p = 0.001), CXCL10 (0.004 vs. 0.42; p = 0.002), and CXCL11 (0.001 vs. 0.023; p = 0.001) in CVF-treated mice 12 days after tumor establishment. *p < 0.05, **p < 0.01, ***p ≤ 0.001