Fig. 5.3.

(a) The normal tether on the C terminus is on the outside of the curved pf, and attaches to the concave membrane on the inside of a liposome or cell. (b) Switching the tether to the N terminus places it on the inside of the curved pf, for attachment to the convex surface on the outside of a liposome. (c) Z rings inside a tubular liposome reconstituted from FtsZ-mts. (d) Inside-out Z rings assembled on the outside of a liposome by mts-FtsZ. In (c) and (d) the upper panel shows FtsZ imaged by fluorescence, and the lower panels show the liposome imaged by DIC (Reprinted from Osawa et al. 2008; Osawa and Erickson 2011 with permission of the authors)