Figure 3. Phylogenetic classification and motif analysis of NAC transcription factors of radish.

Multiple sequence alignment of 172 full-length RsNAC proteins was done using ClustalW, and the phylogenetic tree was mapped using MEGA6 by the neighbor-joining method with 1,000 bootstrap replicates. MEME analysis explored conserved motifs of the RsNAC proteins. Gray lines illustrate the non-conserved sequences and each motif is showed by the colored boxes described at the bottom. The different color background indicated different groups. (A and C) multiple alignment, (B and D) conserved motifs.