TABLE 2.
Primer pairs used within this study
| Gene(s) | Forward primer |
Reverse primer |
Annealing temperature (°C)a | PCR product length (bp) | Reference(s) | ||
|---|---|---|---|---|---|---|---|
| Name | Sequence (5′ to 3′) | Name | Sequence (5′ to 3′) | ||||
| Photosynthetic reaction center (pufL and pufM) | pufL-64F | CTB TTC GAY TTC TGG RTS GG | pufM-754R | CCA TSG TCC AGC GCC AGA A | 55 | 1,600 | 13 |
| pufL | CTK TTC GAC TTC TGG GTS GG | pufM_uniR | CCA TSG TCC AGC GCC AGA A | 60 | 1,600 | 17 | |
| pufL-4F | GCC ATG CTG ARY TTT GAR AAA | pufM-1788R | CTT GAT GGC CCA SAG GTA | 50–61 (52) | 1,800 | This study | |
| Bacteriochlorophyll reductase (bchY) | bchY_fwd | CCN CAR WSN ATG TGY CCN GCN TTY | bchZ_rev2 | ART ABC CSC CNG CNC KRT CRW GRT | 52 | 1,500 | 30, 66 |
| bchY-22F | ACA CGC ACC ATC CCG ATT | bchY-1562R | CAT GWD CCC ATC YCC TCV GMT TT | 50–61 (52) | 1,500 | This study | |
a
The annealing temperature used for PCR is indicated in parentheses.