TABLE 1.
Distribution of cah and cah homologs in Shiga toxin-producing Escherichia colia
| Strain | Serotype | GenBank accession no. |
cah |
cah homologs |
||
|---|---|---|---|---|---|---|
| Gene (positions) or locus tag (corresponding protein length in aa) | % identity | Gene (locus tag or gene positions) (corresponding protein length in aa) | % identity | |||
| EDL933 | O157:H7 | CP008957 | Z1211 (949) | 100 | ND | ND |
| Z1651 (949) | 100 | |||||
| Sakai | O157:H7 | NC_002695 | ECs1396 (949) | 100 | ND | ND |
| TW14359 | O157:H7 | CP001368 | ECSP_1319 (949) | 100 | ND | ND |
| EC4115 | O157:H7 | CP001164 | ECH74115_1395 (949) | 100 | ND | ND |
| Xuzhou21 | O157:H7 | NC_017906 | CDCO157_RS07230 (949) | 100 | ND | ND |
| SS17 | O157:H7 | CP008805 | SS17_4978 (778) | 100 | ND | ND |
| SS52 | O157:H7 | NZ_CP010304 | SS52_1399 (949) | 100 | ND | ND |
| WS4202 | O157:H7 | NZ_CP012802 | AO055_11995 (949) | 100 | ND | ND |
| SRCC 1675 | O157:H7 | CP015023 | AR439_25245 (949) | 100 | ND | ND |
| JEONG-1266 | O157:H7 | CP014314 | JEONG1266_13650 (949) | 100 | ND | ND |
| FRIK944 | O157:H7 | CP016625 | A9L45_07745 (949) | 100 | ND | ND |
| FRIK2069 | O157:H7 | CP015846 | A8V30_07330 (949) | 100 | ND | ND |
| FRIK2455 | O157:H7 | CP015843 | A8V32_07345 (949) | 100 | ND | ND |
| FRIK2533 | O157:H7 | CP015842 | A8V31_07325 (949) | 100 | ND | ND |
| 644-PT8 | O157:H7 | CP015831 | cah (positions 3635954–3633105) (949) | 98.5 | caha (positions 141895–139049) (948) | 89.3 |
| cah (positions 3980603–3977754) (949) | 95.2 | |||||
| 180–PT54 | O157:H7 | CP015832 | cah (positions 3382829–3379980) (949) | 98.5 | caha (positions 141895–139049) (948) | 89.3 |
| cah (positions 3724938–3722089) (949) | 95.2 | |||||
| 28RC1 | O157:H7 | CP015020 | ARC77_26080 (949) | 93.8 | ND | ND |
| 1130 | O157:H7 | NZ_CP017434 | A4C50_17255 (650) | 100 | ND | ND |
| 2149 | O157:H7 | NZ_CP017436 | A4C44_17255 (650) | 100 | ND | ND |
| 2159 | O157:H7 | NZ_CP017438 | A4C45_17280 (650) | 100 | ND | ND |
| 3384 | O157:H7 | NZ_CP017440 | A4C38_17050 (949) | 100 | ND | ND |
| 4276 | O157:H7 | NZ_CP017442 | A4C51_17225 (650) | 100 | ND | ND |
| 8368 | O157:H7 | NZ_CP017444 | A4C39_17025 (949) | 100 | ND | ND |
| 9234 | O157:H7 | NZ_CP017446 | A4C47_17250/(250) | 100 | ND | ND |
| RM13514 | O145:H28 | CP006027 | ECRM13514_1299 (128) | 100 | cahb (ECRM13514_5355) (1,039) | 68.5 |
| RM12581 | O145:H28 | CP007136 | ECRM12581_6385 (128) | 100 | cahb (ECRM12581_26320) (1,039) | 68.5 |
| RM13516 | O145:H28 | CP006262 | ECRM13516_RS06350 (430) | 100 | cahb (ECRM13516_5248) (1,039) | 70.1 |
| cahb (ECRM13516_5033) (1,026) | 69.5 | |||||
| RM12761 | O145:H28 | CP007133 | ECRM12761_6150 (430) | 100 | cahb (ECRM12761_25570) (1,039) | 70.1 |
| cahb ECRM12761_24525 (1,026) | 69.5 | |||||
| 11368 | O26:H11 | NC_013361 | ECO26_1353 (949) | 100 | caha (ECO26_RS17785) (948) | 89.5 |
| cahb (ECO26_RS28925) (1,039) | 68.0 | |||||
| cahb (ECO26_RS15170) (1,039) | 67.9 | |||||
| FORC_028 | O26:H11 | CP012693 | FORC28_2696 (949) | 100 | caha (FORC28_1596) (948) | 89.7 |
| cahb (FORC28_5338) (1,039) | 68.0 | |||||
| cahb (FORC28_2128) (1,039) | 67.9 | |||||
| 08-00022 | O136:H16 | CP013662 | CP48_05340 (949) | 100 | caha (CP48_18180) (948) | 88.0 |
| 09-00049 | O168:H- | CP015228 | GJ12_07155 (949) | 100 | ND | ND |
| 12009 | O103:H2 | NC_013353 | ND | ND | caha (ECO103_RS25970) (948) | 88.4 |
| caha (ECO103_RS18885) (606) | 83.9 | |||||
| 2009EL-2050 | O104:H4 | CP003297 | ND | ND | caha (O3M_00325) (948) | 89.3 |
| cahb (O3M_04255) (1,039) | 68.7 | |||||
| caha (positions 3144849–3146880) (192) | 74.6 | |||||
| 2009EL-2071 | O104:H4 | CP003301 | ND | ND | caha (O3O_25300) (948) | 89.3 |
| cahb (O3O_21435) (1,039) | 68.7 | |||||
| caha (positions 3201209–3203240) (192) | 74.6 | |||||
| 2011C-3493 | O104:H4 | CP003289 | ND | ND | caha (O3K_00310) (948) | 89.3 |
| cahb (O3K_04220) (1,039) | 68.7 | |||||
| caha (positions 3151328–3153359) (192) | 74.6 | |||||
| C227-11 | O104:H4 | CP011331 | ND | ND | caha (AAF13_23370) (948) | 89.3 |
| cahb (AAF13_00880) (1,039) | 68.7 | |||||
| caha (positions 2459518–2461549) (192) | 74.6 | |||||
| 94-3024 | O104:H21 | CP009106 | ND | ND | cahb (HW43_19650) (1,039) | 68.9 |
| 2013C-4465 | O55:H7 | CP015241 | ND | ND | caha (A5955_13015) (948) | 90.0 |
| 06-00048 | O36:H- | CP015229 | ND | ND | caha (GJ11_19470) (948) | 88.8 |
| CFSAN004176 | O145:H- | CP014583 | ND | ND | ND | ND |
| CFSAN004177 | O145:H- | CP014670 | ND | ND | ND | ND |
| 11128 | O111:H- | NC_013364 | ND | ND | ND | ND |
| RM9387 | O104:H7 | NZ_CP009104 | ND | ND | ND | ND |
| 2009C-3133 | O119:H4 | CP013025 | ND | ND | ND | ND |
| 2012C-4227 | O165:H25 | CP013029 | ND | ND | ND | ND |
| GB089 | O168:H- | CP013663 | ND | ND | ND | ND |
| 2011C-3911 | O-:H- | CP015240 | ND | ND | ND | ND |
a
The complete genomes of STEC that were available in GenBank as of September 2016 were used to create a database to search for cah or cah homologs. BLAST was performed in Geneious8.1.8 using EDL933 cah as a query. The retrieved cah and cah homologs were translated using the bacterial translation codon table. The locus tag of the coding DNA sequence (CDS) is based on the original genome annotation. If a locus tag is not available for a cah or cah homolog, the CDS is indicated by the name designated in this study, cah, caha, or cahb, followed by the genomics position of the corresponding gene in parentheses. Items in bold represent genes carrying a mutation in their coding region. The overall mutation rate was 31.3% for cah, 29.4% for caha, and 13.3% for cahb. ND, not detected by BLAST search.