TABLE 2.
Primer name | Sequence | Target | Reference | Amplification efficiency (%) |
---|---|---|---|---|
A915f | GTGCTCCCCCGCCAATTCCT | Archaea | 76 | |
A1059r | GCCATGCACCWCCTCT | Archaea | 77 | 59 |
B340f | TCCTACGGGAGGCAGCAGT | Bacteria | 78 | |
B515r | CGTATTACCGCGGCTGCTGGCAC | Bacteria | 78 | 73 |
ANME-2-240f | CTATCAGGTTGTAGTGGG | ANME-2 | 79 | |
ANME-2-538r | CGGCTACCACTCGGGCCGC | ANME-2 | 79 | 95 |
ANME-1-830r | TCGCAGTAATGCCAACAC | ANME-1 | 79 | |
ANME-1-628f | GCTTTCAGGGAATACTGC | ANME-1 | 79 | 101 |
A1100f | TGGGTCTCGCTCGTTG | Methanomicrobiales | 71 | |
MG1200r | CGGATAATTCGGGGCATGCTG | Methanomicrobiales | 71 | 124 |
MSMX860f | AGGGAAGCCGTGAAGCGCC | Methanosarcinales | 71 | |
A1100r | TGGGTCTCGCTCGTTG | Methanosarcinales | 71 | 51 |