TABLE 2.

Quantitative PCR primers used for taxonomic quantification of 16S rRNA gene

Primer name	Sequence	Target	Reference	Amplification efficiency (%)
A915f	GTGCTCCCCCGCCAATTCCT	Archaea	76
A1059r	GCCATGCACCWCCTCT	Archaea	77	59
B340f	TCCTACGGGAGGCAGCAGT	Bacteria	78
B515r	CGTATTACCGCGGCTGCTGGCAC	Bacteria	78	73
ANME-2-240f	CTATCAGGTTGTAGTGGG	ANME-2	79
ANME-2-538r	CGGCTACCACTCGGGCCGC	ANME-2	79	95
ANME-1-830r	TCGCAGTAATGCCAACAC	ANME-1	79
ANME-1-628f	GCTTTCAGGGAATACTGC	ANME-1	79	101
A1100f	TGGGTCTCGCTCGTTG	Methanomicrobiales	71
MG1200r	CGGATAATTCGGGGCATGCTG	Methanomicrobiales	71	124
MSMX860f	AGGGAAGCCGTGAAGCGCC	Methanosarcinales	71
A1100r	TGGGTCTCGCTCGTTG	Methanosarcinales	71	51