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. 2017 Dec 15;84(1):e01888-17. doi: 10.1128/AEM.01888-17

TABLE 1.

Overall mean populations of Salmonella enterica and EHEC recovered at different sampling points during germination and from different types and tissue sections of sprouts/seedlings

Sampling Mean population (log CFU/g)a
BSA or NASMACb NATSAc
Salmonella
    Trial (n = 256 each)
        1 2.79 A 3.00 A
        2 2.78 A 2.95 A
    Germination day
        9 (n = 128) 3.20 A 3.57 A
        7 (n = 128) 3.19 A 3.40 A
        5 (n = 128) 3.13 A 3.30 A
        3 (n = 96) 1.63 B 1.67 B
        1 (n = 32) 1.45 B 1.48 B
    Strain (n = 128 each)
        S. Stanley 3.09 A 3.52 A
        S. Baildon 3.30 A 3.37 A
        S. Cubana 3.16 A 3.24 A
        S. Montevideo 1.55 B 1.75 B
    Tissue sections of sprouts/seedlings
        Cotyledon (n = 96) 3.63 A 3.82 A
        Seed coat (n = 96) 3.52 A 3.80 A
        Root (n = 128) 2.69 B 2.96 B
        Seed coat/cotyledon (n = 32) 2.11 B,C 2.13 C
        Stem (n = 128) 2.17 C 2.30 C
        Pregerminated seeds (n = 32) 1.45 D 1.48 D
    Vegetable seed type (n = 128 each)
        Fenugreek 4.08 A 4.36 A
        Alfalfa 4.21 A 4.30 A
        Tomato 1.47 B 1.68 B
        Lettuce 1.35 B 1.55 B
EHEC
    Trial (n = 256 each)
        1 1.60 A 1.93 A
        2 1.54 A 1.89 A
    Germination day
        9 (n = 128) 2.30 A 2.91 A
        7 (n = 128) 1.76 B 2.27 B
        5 (n = 128) 1.43 B 1.54 C
        3 (n = 96) 1.11 B,C 1.15 C,D
        1 (n = 32) 0.88 C 1.03 D
    Strain (n = 128 each)
        E. coli K4492 1.70 A 1.91 A
        E. coli H1730 1.72 A 1.93 A
        E. coli BAA-2326 1.49 A,B 1.90 A
        E. coli F4546 1.37 B 1.91 A
    Tissue sections of sprouts/seedlings
        Cotyledon (n = 96) 1.83 A 2.19 A
        Seed coat/cotyledon (n = 32) 1.66 A 2.18 A,B
        Seed coat (n = 96) 1.67 A 1.95 A,B
        Root (n = 128) 1.53 AB 1.85 A,B
        Stem (n = 128) 1.44 AB 1.68 B
        Pregerminated seeds (n = 32) 1.11 B 1.15 C
    Vegetable seed type (n = 128 each)
        Alfalfa 3.31 A 3.70 A
        Fenugreek 1.93 B 2.27 B
        Lettuce 0.67 C 1.54 C
        Tomato 0.38 C 1.15 D
a

Mean populations of the same bacterial pathogen within a column and the same comparative category that are not followed by the same letter are significantly different (P < 0.05).

b

BSA, bismuth sulfite agar; NASMAC, sorbitol MacConkey agar supplemented with nalidixic acid.

c

NATSA, tryptic soy agar supplemented with nalidixic acid.