TABLE 6.

Primers and annealing temperatures used in this study

Gene ID	Target gene	Target forma specialis	Primer name	Primer sequence (5′–3′)	Tann (°C)	Product size (nt)
+	FEM1	Positive control	fp157	ATGAAGTACACTCTCGCTACC	54	274
			fp158	GGTGAAAGTGAAAGAGTCACC
94	HPEG	All cucurbit infecting	fp7304	GCCTCATTGAAGTTTCAACA	54	346
			fp7321	TGGTAAAGGACACGACCATT
13	SIX13	radicis-cucumerinum	fp7305	TTGCCCAAAATGGCATGTTT	56	328
			fp7322	CATTGACACTGTAAGTGGG
70	HPEG	radicis-cucumerinum	fp7306	TACAACCTCTCTCTTTCCTT	54	454
			fp7323	GCTGAATTCTAGCAGAGAAT
66	HPEG	cucumerinum	fp7307	CCGTTATGGCCAGAGATC	54	425
			fp7324	CCAACAAACAGAGCAAAACTAA
99	HPEG	cucumerinum	fp7308	CTACCAATCTCTCCTGAGTG	54	445
			fp7325	GTCGATTGCAGTGCTAGTCT
21	Fom effector 7	cucumerinum	fp7309	CAGTCTAACCCTGTCTCATT	54	381
			fp7326	CGCCAATAGATAGTGATGGA
1	SIX1	melonis	fp7310	CCTCTCAGTCCTTGGGTCT	54	397
			fp7327	ACTCGCTTCAGCTTACCGA
20	Fom effector 6	melonis	fp7406	TGAAAGTCTTGGCGGGTGT	56	305
			fp7328	TCCTCTCCATCCTCATCAGT
18	Fom effector 3	melonis + niveum	fp7312	TTAGTGCAGCTTTTCTCCTC	54	299
			fp7329	AGTGGTTAGTCAAGTGGTAA
99	HPEG	niveum	fp7313	TGCCGGGCTAGTTAATATAGT	54	406
			fp7330	ACCATTTTTCTGTTGGGGTTG
100	HPEG	niveum	fp7314	ATTTTGCTAGCTTCAGCAGTT	54	482
			fp7331	ATCCTGAACGGTGACTAGAG
21	Fom effector 7	niveum	fp7315	CGCTCGCTATAATTCAAACG	54	139
			fp7332	GGAGGAGCACTACAACTAAT
71	HPEG	lagenariae	fp7407	TAGTCCAATCTGCCTCAGCAA	54	270
			fp7410	GGAAGTGAGCATTCTTCCGTA
99	HPEG	lagenariae	fp7408	TCGTATCTCTCAGTAGTATGG	54	367
			fp7411	AATGGATACCTTATAAGGGCT
1	SIX1	lagenariae + momordicae	fp7409	TTGGGATTGCGGCTTATGCT	56	463
			fp7412	AAAGTGGTACACTCCGTGC
98	HPEG	momordicae	fp7318	AGGTGCAGCGTTTTTAGGT	60	469
			fp7335	GAGGGCTGGTTGAGAACTA
130	HPEG	momordicae	fp7319	TCTACGCTTCGAGGATGGTA	56	368
			fp7336	TCGTTTAGACGACTACAACC
99	HPEG	luffae	fp7320	TACTCTCCTAGAGTCAGTCT	54	606
			fp7337	CACGCCATCATCCTTTATTC