TABLE 7.
Primers and probes used for the TaqMan experiments
| Gene ID | Target gene | Target forma specialis | Primer name | Sequence (5′–3′) | Product size (nt) |
|---|---|---|---|---|---|
| 21 | Fom effector 7 | niveum | fp7589 | CCGGTACCCCAGCTTTATGT | 116 |
| fp7590 | CAGCAACGTTCTGAAAGCGT | ||||
| probe_3 | HEX-TGCAGGTTGGCAGGCCCCTG-BHQ1 | ||||
| 100 | HPEG | niveum | fp7591 | CACCAACAACTATGCGGCAC | 138 |
| fp7592 | GCAATTGACCCAGCTGCAAT | ||||
| probe_4 | FAM-AGTCGCCGGCCACCACATTGA-BHQ1 | ||||
| EF1α | Elongation factor 1 α | All strains | fp7710 | CGCTGAGCTCGGTAAGGG | 97 |
| fp7711 | CCAGAGAGCAATATCGATGGTGA | ||||
| probe_7 | TAMRA-ACGCCTGGGTTCTTGACAAGCTCA-BHQ2 |