Figure 3. RBM5 is a direct miR-483-5p target gene in prostate cancer. (A) schematic representation of human RBM5 mRnA; highly conserved miR-483-5p binding sites were located in the 3' UTR. (B) RBM5 levels were examined by western blot analysis. GAPDH was also determined as a loading control. (c) Luciferase assay results from HEK-293T cells co-transfected with the RBM5 3' UTR-wt or RBM5 3' UTR-mut reporter plasmids and either miR-483-5p or miR-NC.

Data are presented as mean±SD from three independent experiments.