Figure 10.

Integrated modeling. ChimeraX window showing starting models, restraints and output from integrative hybrid modeling of the nup84 nuclear pore subcomplex. Hierarchical data organization is shown in upper right including starting comparative models, template structures and sequence alignments for seven components, chemical crosslinking restraints and 2D electron microscopy, and coarse‐grained output ensembles. Graphics shows a best‐scoring model satisfying restraints, large spheres for multi‐residue unordered segments, dashed lines for crosslinks, 2D electron microscopy in background, localization density envelopes for the ensemble of solutions. Lower right panels control electron microscopy rendering and show comparative model and template sequence alignments.