Table 1.

Measures to be administered during stage 2 (Validation) and stage 3 (Wearable Sensor Technology) of the study

		Administered to:
Measure	Key information	Individual with RTT	Individual with ASD	Healthy subjects	Parent/carer of child with RTT/*ASD	Parent/carer/partner of adult with RTT/ASD*	Clinician/researcher
Rett Natural History study32	More than 1000 participants with RTT providing information on important aspects of disorder symptomatology						X
RSBQ38	Provides an accurate measure of the behavioural features of RTT				X	X
RSSS42	Provides information on the overall clinical severity and severity across individual parameters: frequency and manageability of seizures respiratory abnormalities scoliosis ability to walk hand use speech sleep hygiene						X
REST questionnaire	A multidimensional questionnaire that can capture clinically meaningful data across the lifespan in individuals with RTT and improve treatment pathways				X	X	X
Wearable sensor technology	Captures real-time biometric physiological data (heart rate variability, skin conductance, blood volume pressure, perspiration and temperature)	X	X	X
Anticipated administration time (minutes)		30	30	30	~60	~60	~60

*Participants in the ASD cohort will be asked to complete only the relevant questions in the questionnaire battery that would be applicable and relevant to them

ASD, autism spectrum disorder; REST, Rett Evaluation of Symptoms and Treatments Questionnaire; RSBQ, Rett Syndrome Behavioural Questionnaire; RSSS, Rett Syndrome Severity Score; RTT, Rett syndrome.