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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Sci Transl Med. 2017 May 10;9(389):eaal3604. doi: 10.1126/scitranslmed.aal3604

Figure 5. Nivolumab shares similar glycan patterns to mouse aPD-1 and is transferred to macrophages via FcγRs.

Figure 5

(A) HPLC analysis of the glycan patterns found on the mouse aPD-1 mAb and nivolumab shows the G0F isoform to be predominant, but glycosylation is not uniform. (B) AF647-labeled nivolumab (yellow) was used to stain the surface of aCD3 stimulated PKH-green labeled human CD8+ T cells (cerulean) co-incubated with PKH-red labeled peripheral blood mononuclear cell-derived macrophages in the presence or absence of Fc Block. Scale bars represent 20 μm. (C) Quantification of AF647+ puncta per macrophage confirms that nivolumab is transferred via FcγRs. Values represent SEM for 4 separate experiments.