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. 2017 Dec 2;8(1):8–21. doi: 10.1016/j.ijpddr.2017.11.004

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Utilising the INVAPP/Paragon system with ensheathed L3 T. circumcincta. (A) INVAPP/Paragon motility thresholding of single wells treated with DMSO or a DMSO solution of ivermectin (assay concentration 100 μM). Black pixels show those scored as being ‘motile’ by the software. (B) Concentration-response curve for treatment with the anthelmintic ivermectin, n = 4. Each replicate was carried out in different experimental sessions, using one batch of L3 T. circumcincta. Curve fitted using the four-parameter log-logistic function. Error range on the EC₅₀ estimate indicates standard error (delta method).